Journal: Microorganisms

Article Title: Glutamate Transporters GltS, GltP and GltI Are Involved in Escherichia coli Tolerance In Vitro and Pathogenicity in Mouse Urinary Tract Infections

doi: 10.3390/microorganisms11051173

Figure Lengend Snippet: The adhesion and invasion abilities of uropathogenic E. coli UTI89 mutants Δ gltS , Δ gltI , and Δ gltP to bladder epithelial cells. The human epithelial cells (2 × 10 6 cells/well) were infected with E. coli UTI89 and its mutants Δ gltS , Δ gltP , and Δ gltI at a multiplicity of infection (MOI) of 5~10 for 2 h in 24-well plates. Then, the frequencies of bacteria that adhere to and invade the cells were analyzed. The results are expressed as means ± SD. * p < 0.05, *** p < 0.001, relative to E. coli UTI89 group. ( a ) The adhesion rate; ( b ) The invasion rate.

Article Snippet: Human bladder epithelial cells (ATCC HTB-9) were purchased from the Bio-feng company and cultured in RPMI 1640 with 10% newborn calf serum.

Techniques: Infection, Bacteria

Journal: iScience

Article Title: TXNDC12 inhibits lipid peroxidation and ferroptosis

doi: 10.1016/j.isci.2023.108393

Figure Lengend Snippet:

Article Snippet: The human cancer cell lines HL60 (female; CCL-240), K562 (female; CCL-243), MCF7 (female; HTB-22), U2OS (female; HTB-99), PANC1 (male; CRL-1469), HCT116 (male; CCL-247), and SW48 (female; CCL-231) were obtained from the American Type Culture Collection.

Techniques: Recombinant, Lysis, Bicinchoninic Acid Protein Assay, CCK-8 Assay, Transfection, Reporter Assay, Iron Assay, Multiple Displacement Amplification, Magnetic Beads, Mutagenesis, Chromatin Immunoprecipitation, Purification, Enzyme-linked Immunosorbent Assay, shRNA, Software

Journal: bioRxiv

Article Title: Lysine Decarboxylation aids in UPEC intracellular survival in the early stages of urinary tract infection

doi: 10.1101/2025.04.25.650641

Figure Lengend Snippet: Graph shows RT-qPCR results of representative genes from each AR system, using RNA isolated from intracellular bacteria. For this work the bladder epithelial cell line 5637 (ATCC HTB-9) was seeded with wild-type UTI89 at an MOI=10. Following a 2h incubation to allow for pathogen internalization, extracellular bacteria were eliminated by incubating the urothelial cell line with gentamicin for another 2 hours. Gentamicin was then removed and cells were washed 3X with 1X PBS, followed by urothelial cell lysis and subsequent treatment for bacterial lysis and RNA extraction. Extracted RNA was DNAse-treated, reverse-transcribed, quantified and subjected to qPCR using TaqMan probes designed for adiA (purple), cadA (green), gadA (pale blue), gadC (light blue), sdaA (red), sdaC (royal blue), and speF (coral). Transcript abundance was normalized to the gyrB house keeping gene transcripts. Relative fold changes were determined by the ΔΔ C T method, relative to the bacterial inoculum corresponding transcripts. Graph shows analysis of 4 biological replicates. Error bars indicate mean and standard error of the mean at 95% CI.

Article Snippet: Cell culture infections were performed using 5637 human transitional bladder epithelial cells (ATCC HTB-9) originally derived from a 68-year old white male with Grade II bladder carcinoma.

Techniques: Quantitative RT-PCR, Isolation, Bacteria, Incubation, Lysis, RNA Extraction, Reverse Transcription